FY07-09 proposal 200703600
Jump to Reviews and Recommendations
Section 1. Administrative
Proposal title | Mid-Columbia Trophic Dynamics Project |
Proposal ID | 200703600 |
Organization | Washington Department of Fish and Wildlife (WDFW) |
Short description | Conduct a trophics dynamic project using conventional fish capture methods, bioenergetics modeling and stable isotope analysis as well as mobile hydroacoustics surveys to quantify the impacts of predators on salmonids within the Mid-Columbia |
Information transfer | Annual reports will be completed every year at the end of the contract period to present results and inform BPA of project status. Annual reports will also present data, analysis and recommendations should they be required. Annual Reports will be available on line or by request. We will also produce 1 peer review article a year once we begin to collect and analyze data. |
Proposal contact person or principal investigator |
Contacts
Contact | Organization | |
---|---|---|
Form submitter | ||
Dave Burgess | Washington Department of Fish & Wildlife | [email protected] |
All assigned contacts | ||
Dave Burgess | Washington Department of Fish & Wildlife | [email protected] |
Matt Polacek | Washington Department of Fish & Wildlife | [email protected] |
Section 2. Locations
Province / subbasin: Columbia Cascade / Columbia Upper Middle
Latitude | Longitude | Waterbody | Description |
---|---|---|---|
46 9155 | -119.9711 | Middle Upper Columbia River | Mainstem Columbia River from Chief Joseph Dam, downstream to Priest Rapids Dam |
Section 3. Focal species
primary: All Anadromous Fishsecondary: Northern Pikeminnow
Section 4. Past accomplishments
Year | Accomplishments |
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Section 5. Relationships to other projects
Funding source | Related ID | Related title | Relationship |
---|---|---|---|
BPA | 200308700 | Reducing Predation of Salmonid | Project Goal: To quantify the relationship between resident predators and salmonids within the Mid-Columbia River. |
BPA | 199604000 | Coho Restoration Mid-Columbia | Quantifing predation may account for mortality of supplemented salmon as they migrate through the Mid-Columbia River. |
BPA | 200303900 | Monitor Repro In Wenat/Tuc/Kal | Quantifing predation may account for mortality of supplemented salmon as they migrate through the Mid-Columbia River. |
BPA | 200102800 | Banks Lake Fishery Evaluation | Results from this project suggest that a substantial number of non-native fishes are entrained from Banks Lake during the irrigation season. Consequently, Banks Lake as well as other lakes within the basin may ultimately seed the Mid-Columbia with non-native deleterious species. |
BPA | 199502800 | Assessment of Fishery Improvem | Results from this project suggest that a substantial number of non-native fishes are entrained from Moses Lake year round. Consequently, Moses Lake as well as other lakes within the basin may ultimately seed the Mid-Columbia with non-native deleterious species. |
BPA | 199007700 | Dev of Sytemwide Pred Control | Data and results from the Mid-Columbia River mainstem will help with the development and modification of current predator removal program. |
Section 6. Biological objectives
Biological objectives | Full description | Associated subbasin plan | Strategy |
---|---|---|---|
1. Compile and collect pertinent fish data. | Collect necessary data regarding predators and interactions with anadromous and resident fishes. This objective focuses on data collection which will be used to address the Bio. Obj. 4. | Upper Middle Columbia | Bio. Obj. 4. Determine degree of predation by native and non-native species on larval sturgeon. |
2. Influence of predators on Mid-Col. fishes | Apply data collected from biological objectives 2 and conducted analysis regarding rates of consumption and prey selction. | Lower Middle Columbia | Simply listed as predation. Other documents including NPCC draft Columbia River Basin Research Plan suggests studies into predation rates. |
3.impacts of predators on fishes in the Mid-Columb | Data from the previous data will also us to partion prey selection and determine impacts on anadromous and resident fishes. We will also be able relate predation to temporal and spatial parameters. | Lower Middle Columbia | Simply listed as predation. |
4. Reporting and presenting data and results | Data and results will be presented in public and professional forums. Furthermore, we annual reports will be produce for public consumption. | None | The sharing of data and results is pertinent to all subbasin plans. |
Section 7. Work elements (coming back to this)
Work element name | Work element title | Description | Start date | End date | Est budget |
---|---|---|---|---|---|
Collect/Generate/Validate Field and Lab Data | Conduct bi-monthly standardized littoral sampling for predator diets | Using developed standardized sampling protocols we will sample the fishes of a portion of the Mid-Columbia River. To determine the impact on salmonids we will conduct intensive sampling during salmonid outmigrations. | 10/1/2006 | 9/30/2009 | $700,000 |
Biological objectives 1. Compile and collect pertinent fish data. |
Metrics Primary R, M, and E Type: Uncertainties Research Primary R, M, and E Type: Status and Trend Monitoring |
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Collect/Generate/Validate Field and Lab Data | Collect tissue samples for stable isotope analysis (SIA). | Collect tissue samples from predators during sampling events. Tissue samples will be used for stable isotope analysis. | 10/1/2006 | 9/30/2009 | $10,000 |
Biological objectives 1. Compile and collect pertinent fish data. |
Metrics Primary R, M, and E Type: Uncertainties Research |
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Collect/Generate/Validate Field and Lab Data | Collect aging structures from target fishes | Otoliths and scales will be collected from predators during sampling events. This calcified structures will be aged later. | 10/1/2006 | 9/30/2009 | $10,000 |
Biological objectives 1. Compile and collect pertinent fish data. |
Metrics Primary R, M, and E Type: Status and Trend Monitoring |
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Mark/Tag Animals | 1.4 Determine predator abundance | Multiple mark recapture of predators in the Mid-Columbia in order to determine abundance | 10/1/2006 | 9/30/2009 | $90,000 |
Biological objectives 1. Compile and collect pertinent fish data. |
Metrics |
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Analyze/Interpret Data | Perform standard indices analysis from collected field data | Using collected fish data we will determine several standard indices such as relative abundance of fishes, including predators. | 10/1/2006 | 9/30/2009 | $40,000 |
Biological objectives 2. Influence of predators on Mid-Col. fishes |
Metrics Primary R, M, and E Type: Status and Trend Monitoring |
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Collect/Generate/Validate Field and Lab Data | Food habits analysis | Stomach contents collected from predators will be identified in the lab. | 10/1/2006 | 9/30/2009 | $354,000 |
Biological objectives 2. Influence of predators on Mid-Col. fishes |
Metrics Primary R, M, and E Type: Uncertainties Research |
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Analyze/Interpret Data | Perform stable Isotope analysis and modeling. | WDFW staff will prep. samples for SIA. Samples will be sent to the U of I for for quantification of d13C and d15 N. Data will be returned to WDFW project staff at whch time a trophic web will be created as well as data applied to a Isotope (Isoconc 1.01) mixing model. | 10/1/2006 | 9/30/2009 | $80,000 |
Biological objectives 2. Influence of predators on Mid-Col. fishes |
Metrics Primary R, M, and E Type: Uncertainties Research |
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Analyze/Interpret Data | Determine age structures of predators | Collected scales and otoliths will be aged at the WDFW aging lab. We will also perform length frequency analysis to pair with aging results. | 10/1/2006 | 9/30/2009 | $20,000 |
Biological objectives 2. Influence of predators on Mid-Col. fishes |
Metrics Primary R, M, and E Type: Uncertainties Research |
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Analyze/Interpret Data | Bioenergetics modeling | Proportions of diets, temperature and growth data collected from predators will be used to calculate predator consumption rates using a Fish Bioenergetics model 3.0. | 10/1/2006 | 9/30/2009 | $30,000 |
Biological objectives 2. Influence of predators on Mid-Col. fishes |
Metrics Primary R, M, and E Type: Uncertainties Research |
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Analyze/Interpret Data | Impacts on anadromous fishes | Assuming we can obtain accurate smolt migration counts between dams we will be able to estimate what proportion of mortality is attributed to predation. Examining smolt counts from one dam to the next will provide us with an estimate of the number of anadromous fishes mortalities. For example, if one million smolts passed Dam 1 and 800,000 were observed passing next nearest dam then an estimate of loss for the reach between the two dams would be 200,000 smolts or 20%. We will then use bioenergetics modeling results (Work Element Title 3.5) to determine what percentage of overall mortality can be attributed to resident predators. | 12/1/2006 | 9/30/2009 | $20,000 |
Biological objectives 3.impacts of predators on fishes in the Mid-Columb |
Metrics Primary R, M, and E Type: Uncertainties Research |
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Analyze/Interpret Data | Determine impacts on resident fishes | Secondary data from Work Element Titles 2.1, 2.2, and 2.3 while investigating impacts on salmonids will allow us to quantify the potential impact predators may be having on sensitive resident fishes such as white sturgeon, bulltrout (Salvalinus confluentus) and possibly lamprey. | 12/1/2006 | 9/30/2009 | $20,000 |
Biological objectives 3.impacts of predators on fishes in the Mid-Columb |
Metrics Primary R, M, and E Type: Uncertainties Research |
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Analyze/Interpret Data | Relating predation to temporal and spatial parameters. | Data from Work Element Titles 3.2 and 3.3 associated with the geographical location of where predators were captured will permit us to determine when and where predation takes place and at what level. Information from this Work Element will be input into a GIS to produce a spatial representation of predation. | 1/1/2007 | 9/30/2009 | $50,000 |
Biological objectives 3.impacts of predators on fishes in the Mid-Columb |
Metrics Primary R, M, and E Type: Uncertainties Research |
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Produce/Submit Scientific Findings Report | Pices reporting | Produce Status Report (141) Quarterly reporting to BPA regarding the status of the project. These reports will also include data and analysis when applicable. | 1/1/2007 | 9/30/2009 | $10,000 |
Biological objectives 4. Reporting and presenting data and results |
Metrics |
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Produce/Submit Scientific Findings Report | Quarterly reporting | Quarterly reporting to BPA regarding the status of the project. These reports will also include data and analysis when applicable. | 1/1/2007 | 9/30/2009 | $20,000 |
Biological objectives 4. Reporting and presenting data and results |
Metrics |
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Produce/Submit Scientific Findings Report | Annual Reports | Annual reports will be completed every year at the end of the contract period to present results and inform BPA of project status. Annual reports will also present data, analysis and recommendations should they be required. This will also be the appropriate time to request budget modifications or changes within the work plan. | 10/1/2006 | 9/30/2009 | $200,000 |
Biological objectives 4. Reporting and presenting data and results |
Metrics |
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Outreach and Education | Stakeholder presentations | WDFW and BPA of our results but also the many concerned stakeholders that have personal and professional interests in our project. Consequently, some time will be spent presenting our information in public forums. These presentations will include the data and results contained within our annual reports. | 10/1/2006 | 9/30/2009 | $5,000 |
Biological objectives 4. Reporting and presenting data and results |
Metrics * # of general public reached: One annual presentation |
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Manage and Administer Projects | Additional administrative duties | This task will include the day-to-day operations associated with project personnel, agency policy, purchases, additional inter- and intra-agency exercises and budget monitoring. | 10/1/2006 | 9/30/2009 | $30,000 |
Biological objectives 4. Reporting and presenting data and results |
Metrics |
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Produce Environmental Compliance Documentation | Obtain necessary sampling and collection permits | Because we will be sampling in an area with anadromous fishes we will be required to obtain the necessary state and federal permits to do so. We are currently working with the WDFW environmental compliance/permit advisor obtain a section 10 as well as the state’s blanket sampling permit. | 10/1/2006 | 9/30/2009 | $10,000 |
Biological objectives 4. Reporting and presenting data and results |
Metrics |
Section 8. Budgets
Itemized estimated budget
Item | Note | FY07 | FY08 | FY09 |
---|---|---|---|---|
Personnel | 2 Biologists, 1 perm. techs, 6 temp techs. | $236,000 | $236,000 | $236,000 |
Fringe Benefits | Medical, SS, L&I | $78,000 | $78,000 | $78,000 |
Supplies | 95% Reagent Alcohol, whirl pacs, lab supplies, field safety gear, GPS units, misc. field supplies, 2 microscopes, computers and software, thermographs, GSA truck lease. | $50,000 | $50,000 | $50,000 |
Travel | Per Diem, lodging, fuel. | $35,000 | $35,000 | $35,000 |
Capital Equipment | 22' electrofishing boat and gear | $100,000 | $0 | $0 |
Overhead | 29.3% | $120,000 | $120,000 | $120,000 |
Other | University of Idaho SIA, Contract wiit Dr. David Bennett | $14,000 | $14,000 | $14,000 |
Totals | $633,000 | $533,000 | $533,000 |
Total estimated FY 2007-2009 budgets
Total itemized budget: | $1,699,000 |
Total work element budget: | $1,699,000 |
Cost sharing
Funding source/org | Item or service provided | FY 07 est value ($) | FY 08 est value ($) | FY 09 est value ($) | Cash or in-kind? | Status |
---|---|---|---|---|---|---|
Totals | $0 | $0 | $0 |
Section 9. Project future
FY 2010 estimated budget: $0 FY 2011 estimated budget: $0 |
Comments: Future costs can not be determined as future work elements will be developed based on the analysis and recommendations of Phase 1. |
Future O&M costs: Our study plan is in accordance with the Fish and Wildlife Program and will use “experimental designs and techniques as part of management actions and integrating monitoring and research with those management actions to evaluate their effects on the ecosystem”(NPPC 2000, pg 13). The first phase of the project will address the RM&E Framework Component, Predator Status and Trend Monitoring, which poses the management question; what is the impact of predators on juvenile salmonids within the Columbia River (NPCC 2005 draft Columbia River Basin Research Plan). Should negative interactions exist, Phase 2 will explore measures to alleviate such interactions. If management recommendations to reduce predator impacts are deemed feasible then Phase 3 will be conducted which calls for the implementation of predator reduction actions. Each Phase of this project has project goals. Monitoring and evaluation will continue to gauge the success of such programs.
Termination date:
Comments:
Final deliverables:
Section 10. Narrative and other documents
revised 200703600n | Jul 2006 |
Reviews and recommendations
FY07 budget | FY08 budget | FY09 budget | Total budget | Type | Category | Recommendation |
---|---|---|---|---|---|---|
NPCC FINAL FUNDING RECOMMENDATIONS (Oct 23, 2006) [full Council recs] | ||||||
$0 | $0 | $0 | $0 | Expense | Multi-province | Do Not Fund |
NPCC DRAFT FUNDING RECOMMENDATIONS (Sep 15, 2006) [full Council recs] | ||||||
$0 | $0 | $0 | $0 | Multi-province | ||
Comments: Reduced or removed due to ISRP concerns. MSRT recommends $0. |
ISRP PRELIMINARY REVIEW (Jun 2, 2006)
Recommendation: Not fundable
NPCC comments: The proposal in its present form is not fundable. This is a proposal to develop a research, monitoring, and evaluation plan. The proposed location-specific information on predators and predation rates on salmonids in the Mid-Columbia would be both more up-to-date and local than existing information from the lower Columbia. A better understanding of the impacts of the predators is warranted but this proposal is not sufficiently justified to address this data gap. Particularly, the methods are insufficiently described. If the proposal focused on developing a method to estimate predator population size and food habits, it could be developed into a fundable project. Additional comments by ISRP reviewers are listed below. Technical and scientific background: Scientific and technical information related to the Columbia River Basin is adequately explained with references. This section of the proposal is brief, and would have benefited from a brief review of relevant studies in other geographic regions. Rationale and significance to subbasin plans and regional programs: The potential significance of predators is noted in the subbasin plans for the project area and is generally recognized as a problem in the basin. The proponents mention subbasin plans and Council's research plan, but do not make a strong case for whether predator trophic dynamics studies are a high priority in these plans. Relationships to other projects: The proponents provide a description of how their results will be applicable to several ongoing and newly proposed studies. They plan to work with the Chelan County and Grant County Public Utility Districts’ pikeminnow removal programs to collect additional stomach and tissue samples if required. Objectives: The first objective in which the proponents want to do the project planning with project funding suggests that this proposal is incomplete; i.e., this is a proposal to do a proposal. The general objective to improve the understanding of predatory fishes’ impact on migrating salmonids is very appropriate. However, the relationships among the specific objectives listed in the proposal are not clear. For example, in work element 3.1 the statement is made that a population estimate of predators will not be possible. However, much of the sampling effort (e.g., littoral sampling, hydroacoustic sampling) seems to be focused on developing some understanding of predator abundance. In fact, without a good estimate of predator abundance, it will not be possible to estimate impacts on migrating salmon, even with the use of the bioenergetics model. One of the primary objectives of this work should be developing a methodology that will provide an estimate of predator abundance. The rationale for collecting the water quality data is unclear. Developing a relationship between water quality attributes and salmonid susceptibility to predation would require sampling at frequent intervals, across a range of water quality conditions, at at least one site within each of the broad habitat classes (forebay, tailrace, reservoir). However, sampling will occur only in spring and fall. Therefore, only two points per site, per year will be collected. The possibility of developing a meaningful understanding of the relationship between water quality and predation rate seems pretty remote given the paucity of the data. The objectives at the end are presuming good results and go on to actions, which are acceptable for projecting ahead, but are premature for inclusion in this proposed work. The proposed timelines for the three phases of the project are not clear. Tasks (work elements) and methods: Many of the work elements are not clearly described. This point clearly applies to the issue of estimating predator population size, mentioned above. Much more thought needs to be given to this aspect of the study. In fact, the usefulness of the information collected in this project would be severely compromised unless some estimate of population size is made. The use of multiple sampling techniques to enumerate predator populations exacerbates the problem. Is it possible to combine data collected during the littoral sampling with the hydroacoustic data? The issue of data compatibility is especially problematic given that the littoral data is collected in the spring and fall and the hydroacoustic data in mid summer. It might be worthwhile to consider restricting the sampling effort to a much smaller section of the river (between two dams, maybe) and concentrate on developing a solid estimate of salmonid losses to predation at this site. Subsequently, the methodology could be applied to other locations. The proponent’s description of Phase 2 indicates that evaluation of any predator control strategy will require monitoring of predator population size. This point further emphasizes the need to develop a method for measuring population size. It is not clear what types of samples (other than fish muscle) will be used in the stable isotope analysis. In order to construct a food web for the system, samples of all the major food items of the predatory fishes need to be collected for isotopic analysis. Without these data, it will not be possible to draw any conclusions about the diets of the predators beyond what you learn from the gut contents. If samples of food items are planned, this should have been described in the proposal. If there is no plan to collect samples of food items, the stable isotope analysis should be omitted from the study. Work element 1.1: This is a proposal to do a literature search to further develop research, monitoring, and evaluation methods and a sample design. In general, the proposal would have been improved if this work had been completed prior to submission of the proposal. Work element 1.2: Standard WDFW protocols for selecting samples sites will be used. Methods of random site selection are not described. Sampling methods to be used include gillnetting, electrofishing, fykenetting, and angling but no details on gear (e.g., mesh size), fishing methods, or fishing strategy with respect to target species are provided. Additional habitat types will be designated depending on gear types. The proposal would have been improved if the study area and sampling design had been completed and included as part of the proposal. Work element 2.1: The proponents would limit sampling to the spring to "when smolt are migrating" and fall "to ascertain diet data associated with smolt absence." Why isn't predation on juvenile salmon parr, which might be rearing and feeding in reservoirs throughout the year, of interest in this study? Part of the description of hydroacoustic methods is written in past tense - is this methodology derived from another study conducted by the proponents? Again, the proposal would be improved by a description of the net sampling gear and procedures that would be used to validate species composition and size distribution. Throughout the proposal, statistical data analysis procedures -- sample sizes/statistical power -- are not provided. Work elements for phase II and III of the proposal are not fully developed. Monitoring and evaluation: This is a monitoring and evaluation project. However, there are deficiencies in the design that should be addressed before the project is implemented. Facilities, equipment, and personnel: The budget request includes a new boat, a new truck and several other capital items. These needs suggest that current equipment and facilities may not be sufficient to undertake this project. Where will the stable isotope analyses work be done? Only the lead staff person's CV was given, and a few other names are listed in the text as writers. We have to presume that the state staff knows how to do the planned work. What are the roles of Polacek, Simmons, Bennett, and Schroder in the proposed study? Information transfer: The public outreach component is especially noteworthy. The proposal would have been improved if plans for publication of results in a scientific journal were included. Plans for release and long-term storage of data and meta-data are not described. Benefits to focal species and non-focal species: The proposed project is intended to benefit salmon populations through predator control (if warranted by the results), but it is not demonstrated that benefits would be significant or persist over the long term. Without a good estimate of predator population levels, the impact of these fishes on migrating salmon cannot be estimated and the effectiveness of any predator control strategy that is implemented cannot be assessed. This problem reduces the benefit of this project to the focal species. Knowing more about the predatory fishes and the consumption of salmon will likely benefit salmon populations, but there is some uncertainty. There are potential adverse effects of the sampling (e.g., electrofishing) on salmonids and other species of native biota. Any predator control program implemented as a result of this work could have unforeseen impacts on aquatic communities in the mainstem. However, it would seem unlikely that these impacts would be to species that are the primary targets of recovery efforts. There likely will be some beneficial information gathered on species other than the major predator and prey species that are being targeted.
ISRP FINAL REVIEW (Aug 31, 2006)
Recommendation: Not fundable
NPCC comments: Even with the response, this remains a plan to develop a plan. The ISRP's earlier recommendation of "Not fundable" stands. The ISRP's preliminary comments (June 1, 2006): The proposal in its present form is not fundable. This is a proposal to develop a research, monitoring, and evaluation plan. The proposed location-specific information on predators and predation rates on salmonids in the Mid-Columbia would be both more up-to-date and local than existing information from the lower Columbia. A better understanding of the impacts of the predators is warranted but this proposal is not sufficiently justified to address this data gap. Particularly, the methods are insufficiently described. If the proposal focused on developing a method to estimate predator population size and food habits, it could be developed into a fundable project. Additional comments by ISRP reviewers are listed below. Technical and scientific background: Scientific and technical information related to the Columbia River Basin is adequately explained with references. This section of the proposal is brief, and would have benefited from a brief review of relevant studies in other geographic regions. Rationale and significance to subbasin plans and regional programs: The potential significance of predators is noted in the subbasin plans for the project area and is generally recognized as a problem in the basin. The proponents mention subbasin plans and Council's research plan, but do not make a strong case for whether predator trophic dynamics studies are a high priority in these plans. Relationships to other projects: The proponents provide a description of how their results will be applicable to several ongoing and newly proposed studies. They plan to work with the Chelan County and Grant County Public Utility Districts’ pikeminnow removal programs to collect additional stomach and tissue samples if required. Objectives: The first objective in which the proponents want to do the project planning with project funding suggests that this proposal is incomplete; i.e., this is a proposal to do a proposal. The general objective to improve the understanding of predatory fishes’ impact on migrating salmonids is very appropriate. However, the relationships among the specific objectives listed in the proposal are not clear. For example, in work element 3.1 the statement is made that a population estimate of predators will not be possible. However, much of the sampling effort (e.g., littoral sampling, hydroacoustic sampling) seems to be focused on developing some understanding of predator abundance. In fact, without a good estimate of predator abundance, it will not be possible to estimate impacts on migrating salmon, even with the use of the bioenergetics model. One of the primary objectives of this work should be developing a methodology that will provide an estimate of predator abundance. The rationale for collecting the water quality data is unclear. Developing a relationship between water quality attributes and salmonid susceptibility to predation would require sampling at frequent intervals, across a range of water quality conditions, at least one site within each of the broad habitat classes (forebay, tailrace, reservoir). However, sampling will occur only in spring and fall. Therefore, only two points per site, per year will be collected. The possibility of developing a meaningful understanding of the relationship between water quality and predation rate seems pretty remote given the paucity of the data. The objectives at the end are presuming good results and go on to actions, which are acceptable for projecting ahead, but are premature for inclusion in this proposed work. The proposed timelines for the three phases of the project are not clear. Tasks (work elements) and methods: Many of the work elements are not clearly described. This point clearly applies to the issue of estimating predator population size, mentioned above. Much more thought needs to be given to this aspect of the study. In fact, the usefulness of the information collected in this project would be severely compromised unless some estimate of population size is made. The use of multiple sampling techniques to enumerate predator populations exacerbates the problem. Is it possible to combine data collected during the littoral sampling with the hydroacoustic data? The issue of data compatibility is especially problematic given that the littoral data is collected in the spring and fall and the hydroacoustic data in mid summer. It might be worthwhile to consider restricting the sampling effort to a much smaller section of the river (between two dams, maybe) and concentrate on developing a solid estimate of salmonid losses to predation at this site. Subsequently, the methodology could be applied to other locations. The proponent’s description of Phase 2 indicates that evaluation of any predator control strategy will require monitoring of predator population size. This point further emphasizes the need to develop a method for measuring population size. It is not clear what types of samples (other than fish muscle) will be used in the stable isotope analysis. In order to construct a food web for the system, samples of all the major food items of the predatory fishes need to be collected for isotopic analysis. Without these data, it will not be possible to draw any conclusions about the diets of the predators beyond what you learn from the gut contents. If samples of food items are planned, this should have been described in the proposal. If there is no plan to collect samples of food items, the stable isotope analysis should be omitted from the study. Work element 1.1: This is a proposal to do a literature search to further develop research, monitoring, and evaluation methods and a sample design. In general, the proposal would have been improved if this work had been completed prior to submission of the proposal. Work element 1.2: Standard WDFW protocols for selecting samples sites will be used. Methods of random site selection are not described. Sampling methods to be used include gillnetting, electrofishing, fykenetting, and angling but no details on gear (e.g., mesh size), fishing methods, or fishing strategy with respect to target species are provided. Additional habitat types will be designated depending on gear types. The proposal would have been improved if the study area and sampling design had been completed and included as part of the proposal. Work element 2.1: The proponents would limit sampling to the spring to "when smolt are migrating" and fall "to ascertain diet data associated with smolt absence." Why isn't predation on juvenile salmon parr, which might be rearing and feeding in reservoirs throughout the year, of interest in this study? Part of the description of hydroacoustic methods is written in past tense - is this methodology derived from another study conducted by the proponents? Again, the proposal would be improved by a description of the net sampling gear and procedures that would be used to validate species composition and size distribution. Throughout the proposal, statistical data analysis procedures -- sample sizes/statistical power -- are not provided. Work elements for phase II and III of the proposal are not fully developed. Monitoring and evaluation: This is a monitoring and evaluation project. However, there are deficiencies in the design that should be addressed before the project is implemented. Facilities, equipment, and personnel: The budget request includes a new boat, a new truck and several other capital items. These needs suggest that current equipment and facilities may not be sufficient to undertake this project. Where will the stable isotope analyses work be done? Only the lead staff person's CV was given, and a few other names are listed in the text as writers. We have to presume that the state staff knows how to do the planned work. What are the roles of Polacek, Simmons, Bennett, and Schroder in the proposed study? Information transfer: The public outreach component is especially noteworthy. The proposal would have been improved if plans for publication of results in a scientific journal were included. Plans for release and long-term storage of data and meta-data are not described. Benefits to focal species and non-focal species: The proposed project is intended to benefit salmon populations through predator control (if warranted by the results), but it is not demonstrated that benefits would be significant or persist over the long term. Without a good estimate of predator population levels, the impact of these fishes on migrating salmon cannot be estimated and the effectiveness of any predator control strategy that is implemented cannot be assessed. This problem reduces the benefit of this project to the focal species. Knowing more about the predatory fishes and the consumption of salmon will likely benefit salmon populations, but there is some uncertainty. There are potential adverse effects of the sampling (e.g., electrofishing) on salmonids and other species of native biota. Any predator control program implemented as a result of this work could have unforeseen impacts on aquatic communities in the mainstem. However, it would seem unlikely that these impacts would be to species that are the primary targets of recovery efforts. There likely will be some beneficial information gathered on species other than the major predator and prey species that are being targeted.