FY 2002 Blue Mountain proposal 199801006

Section 1. Administrative

Proposal titleCaptive Broodstock Artificial Propagation
Proposal ID199801006
OrganizationNez Perce Tribe Department of Fisheries Resources Management (NPT)
Proposal contact person or principal investigator
NameJames R. Harbeck
Mailing address612 SW 2nd Enterprise, OR 97828
Phone / email5414263198 / [email protected]
Manager authorizing this projectJaime Pinkham
Review cycleBlue Mountain
Province / SubbasinBlue Mountain / Grande Ronde
Short descriptionImplement and evaluate the captive broodstock project through the collection of juvenile salmon from the wild and maintaining them in captivity. The founding generation is spawned and the resulting F1 generation is released back to the parental stream.
Target speciesSpring chinook salmon (Oncorhynchus tshawytscha)
Project location
LatitudeLongitudeDescription
45.422 -117.2775 NPT Enterprise Field office
45.5521 -117.49 Lostine River
45.3139 -117.8722 Catherine Creek
45.52 -117.76 Upper Grande Ronde
45.7314 -117.864 Lookingglass Fish Hatchery
45.6323 -121.956 Bonneville Hatchery
47.5042 -122.835 Manchester Marine Laboratory
45.6756 -121.853 Oxbow Hatchery
45.9062 -119.4951 Irrigon Hatchery
Reasonable and Prudent Alternatives (RPAs)

Sponsor-reported:

RPA
Hatchery RPA Action 176
RM&E RPA Action 184
RM&E RPA Action 185
RM&E RPA Action 193
Hydro RPA Action 118

Relevant RPAs based on NMFS/BPA review:

Reviewing agencyAction #BiOp AgencyDescription
NMFS/BPA Action 177 NMFS In 2002, BPA shall begin to implement and sustain NMFS-approved, safety-net projects.

Section 2. Past accomplishments

YearAccomplishment
1995 Cooperatively developed with coomanagers the application for a Section 10 Permit in order to collect chinook parr from the Lostine, Catherine Creek and upper Grande Ronde Rivers.
1996 Participated in CONSPOT and captive broodstock management meetings.
1997 Cryopreserved semen from 63 captive broodstock males.
1998 Acquired funding for full NPT participation in the captive broodstock program.
1998 Recruited project leader for the program.
1998 Collected 501 wild parr from the Lostine River.
1998 PIT tagged and collected data from wild juvenile captives at LGH.
1998 Implanted VI tags and collected data from immature and maturing captives at BOH and MML.
1998 Spawned 317 captive broodstock (122 females).
1998 Cryopreserved semen from 108 captive broodstock males.
1998 Prepared quarterly results and presented results.
1999 Inventoried existing captive brood cryopreserved semen collections at BOH and Frontier Genetics.
1999 Collected 498 wild parr from the Lostine River.
1999 PIT tagged and collected data from wild juvenile captives at LGH.
1999 Implanted VI tags and collected data from immature and maturing captives at BOH and MML.
1999 Spawned 665 captive broodstock (307 females).
1999 Cryopreserved semen from 207 captive broodstock males.
1999 Prepared quarterly results.
1999 Recruited for a captive broodstock technician.
2000 Collected 500 wild parr from the Lostine River.
2000 PIT tagged and collected data from wild juvenile captives at LGH.
2000 Implanted VI tags and collected data from immature and maturing captives at BOH and MML.
2000 Spawned 1,037 captive broodstock (458 females).
2000 Cryopreserved semen from 9 captive broodstock males.
2000 Thawed and utilized cryopreserved semen from 57 males in spawning matrices.
2000 Determined fertilization rates for females fertilized with fresh and cryopreserved semen.
2000 Acclimated and released captive progeny F1 smolts.
2000 Monitored downstream migration and compared performance between F1 and wild smolts
2000 Prepared quarterly results and presented results.
2001 Implanted VI tags and collected data from immature and maturing captives at BOH and MML.
2001 Acclimated and released captive progeny F1 smolts.
2001 Monitored downstream migration and compared performance between F1 and wild smolts.
2001 Monitored adult captive progeny return to parental streams.

Section 3. Relationships to other projects

Project IDTitleDescription
199801001 Grande Ronde Basin Spring Chinook Captive Broodstock Program Depends on project 199801001 to rear smolt-to-adult at Bonneville Hatchery (freshwater strategy).
199606700 Manchester Captive Brood O&M Depends on project 199606700 to rear smolt-to-adult at Manchester Marine Laboratory (saltwater strategy).
199305600 Assessment of Captive Broodstock Technology Depends on project 199305600 for NMFS assessment of captive broodstock technology.
199703800 Listed Stock Chinook Salmon Gamete Preservation Depends on project 199703800 for the use of the regional gene repository located at the University of Idaho.
199800702 Grand Ronde Supplementation Project O&M/M&E Is integrated with the activities of project 199800702. The Lostine component monitors conventional production.
198805301 Northeast Oregon Hatcheries Will be integrated with the activities of project 198805301. NEOH will provide a coordinated umbrella to all supplementation projects in northeast Oregon.
198712700 Grande Ronde Smolt Monitoring Depends on project 198712700 for smolt trapping assistance on the Lostine River. Will provide natural survival performance standards.
Lower Snake River Compensation Plan Depends on LSRCP for parr-to-smolt rearing at Lookingglass Hatchery

Section 4. Budget for Planning and Design phase

Task-based budget
ObjectiveTaskDuration in FYsEstimated 2002 costSubcontractor
Outyear objectives-based budget
ObjectiveStarting FYEnding FYEstimated cost
Outyear budgets for Planning and Design phase

Section 5. Budget for Construction and Implementation phase

Task-based budget
ObjectiveTaskDuration in FYsEstimated 2002 costSubcontractor
Outyear objectives-based budget
ObjectiveStarting FYEnding FYEstimated cost
Outyear budgets for Construction and Implementation phase

Section 6. Budget for Operations and Maintenance phase

Task-based budget
ObjectiveTaskDuration in FYsEstimated 2002 costSubcontractor
Outyear objectives-based budget
ObjectiveStarting FYEnding FYEstimated cost
Outyear budgets for Operations and Maintenance phase

Section 7. Budget for Monitoring and Evaluation phase

Task-based budget
ObjectiveTaskDuration in FYsEstimated 2002 costSubcontractor
1. Coordinate the Captive Broodstock Artificial Propagation project with state and federal management agencies in the Grande Ronde Basin. a. Coordinate all aspects of the Grande Ronde Basin chinook captive broodstock project planning, implementation and monitoring and evaluation with ODFW, NMFS and CTUIR. on-going $3,000
b. Attend TOT meetings to represent the interst of and to provide information for the Nez Perce Tribe. on-going $4,000
c. Coordinate and facilitate the use of collected data with co-managers. on-going $3,000
d. Assist in computer database management of all monitoring information collected on chinook captive broodstock at LFH, MML, and BOH. on-going $4,000
2. Monitor and evaluate captive broodstock parr reared at Lookingglass Fish Hatchery (LFH) with ODFW and CTUIR. a. Collect chinook parr from the Lostine River during August. on-going $4,000
b. Assist in loading and transport of juvenile chinook to LFH and determine the mortality rate of juvenile chinook collected and transported to LFH and collect information on observed mortalities. on-going $2,000
c. Assist in PIT tagging all captive brood juvenile chinook in LFH. on-going $2,000
d. Collect biological information of fork length, weight and general fish condition from a sample of fish at LFH at regular periodic intervals and assess growth profiles and condition factors of chinook captive broodstock at LFH. on-going $1,000
e. Collect caudal tissue from juvenile chinook for genetic analysis. on-going $1,000
f. Determine survival rate of juvenile fish in LFH from collection to transport off-station to the MML and BOH. on-going $1,000
3. Monitor and evaluate captive broodstock post smolts reared at Bonneville Hatchery and at the Manchester Marine Laboratory. a. Tag fish with visual implant (VI) tags and measure fork length and weight of a sample of the fish. on-going $3,000
b. Measure fork length and weight of a sample of fish when any rearing parameters change. on-going $3,000
c. Assess growth profiles of chinook captive broodstock at Manchester Marine Laboratory and at Bonneville Hatchery. on-going $3,000
d. Determine survival rates of chinook captive broodstock under saltwater rearing (MML) and freshwater (BOH) rearing strategies. on-going $3,000
e. Determine maturation rates, ages II through V, of chinook captive broodstock under saltwater rearing (MML) and freshwater (BOH) rearing strategies. on-going $3,000
f. Assist in spawning freshwater reared and saltwater reared chinook captive broodstock adults at BOH. on-going $12,000
g. Collect caudal tissue from adult chinook for genetic analysis. on-going $4,000
h. Coordinate the cryopreservation activities associated with captive broodstock spawning. on-going $7,096
i. Collect fork length and weight information on all spawned fish. on-going $4,000
j. Compare the age and size at maturity of adults that are reared in freshwater versus saltwater. on-going $4,000
k. Determine and compare the fecundity of females reared in freshwater versus saltwater rearing strategies. on-going $4,000
l. Determine and compare fertilization rates for all spawned females in freshwater reared and saltwater rearing strategies. on-going $4,500
m. Determine and compare the timing of spawning for freshwater reared and saltwater reared captive adults. on-going $4,581
n. Analyze collected genetic tissues on-going $12,000 Yes
4. Monitor and evaluate the F1 generation juveniles. a. Calculate and compare green-egg to eyed-egg survival between treatment groups. on-going $4,000
b. Measure and compare growth profiles between treatment groups. on-going $4,000
c. Assist ODFW in Pit tagging a subsample of F1 generation parr at LGH and determine mark and tag efficiency/retention. on-going $26,000
d. Monitor the volitional release of F1 smolts from the Lostine River Acclimation Facility. on-going $4,000
e. Assist ODFW with the operation of the Lostine screw trap to monitor the migration of the captive F1 smolts and compare with wild smolt emigration performance. on-going $7,000
f. Estimate arrival timing, detection rate and survival of released F1generation smolts to Lower Granite, Little Goose, Lower Monumental and McNary Dams Survival and compare with wild smolt emigration performance. Survival is estimated with SURPH.2. on-going $4,000
5. Monitor and evaluate the captive F1 generation adults. a. Install the adult fish weir and trapping structure on the Lostine River as soon as water discharge and velocity criteria allow. on-going $500
b Assist in trap operation and collection of information on adult salmon as needed. on-going $0
c. Coordinate and gather information collected on abundance and timing of the adult salmon spawner migration into the Lostine River. on-going $1,000
d. Determine the abundance, sex ratio and timing of wild adult salmon and compare with the captive F1 adult progeny contributing to the chinook run in the Lostine River. on-going $1,000
e. Compare the adult spawner migration into Lostine River with stream discharge and water temperature and examine correlations between these variables over time. on-going $1,000
f. Assist ODFW with annual chinook salmon spawning ground surveys to determine adult size, age composition, stray rates and female egg retention from carcasses sampled in the Lostine River and compare wild and captive adult progeny. on-going $5,000
g. Assist in removal of the fish weir and trap by October 1 or after the adult spawner migration is finished. . on-going $500
6. Technology Transfer a. Prepare and provide BPA quarterly reports summarizing activities accomplished during the quarter. on-going $5,000
b. Prepare and provide annual reports cooperatively with ODFW and CTUIR summarizing all activities associated with the chinook salmon captive broodstock project. on-going $6,000
c. Present reports on project activities and findings at Annual BPA/CBFWA Project Review and other forums (i.e., AFS, NAFWS, LSRCP Annual Review). on-going $5,000
Outyear objectives-based budget
ObjectiveStarting FYEnding FYEstimated cost
1. Coordinate the Captive Broodstock Artificial Propagation project with state and federal management agencies in the Grande Ronde Basin. 2003 2006 $60,328
2. Monitor and evaluate chinook captive broodstock reared at Lookingglass Fish Hatchery (LFH) with ODFW and CTUIR. 2003 2006 $47,401
3. Monitor and evaluate chinook captive broodstock reared at Bonneville Hatchery and at the Manchester Marine Laboratory. 2003 2006 $305,949
4 &5. Monitor and evaluate the F1 generation offspring. 2003 2006 $237,003
6. Technology Transfer 2003 2006 $68,946
Outyear budgets for Monitoring and Evaluation phase
FY 2003FY 2004FY 2005FY 2006
$175,282$180,541$185,957$191,537

Section 8. Estimated budget summary

Itemized budget
ItemNoteFY 2002 cost
Personnel FTE: 1.75 $59,806
Fringe 35%/25% $20,057
Supplies $15,500
Travel $17,273
Indirect 20.9% $23,541
Capital Administrative Trailer $6,000
PIT tags # of tags: 8,000 $18,000
Subcontractor genetic analysis $10,000
$170,177
Total estimated budget
Total FY 2002 cost$170,177
Amount anticipated from previously committed BPA funds$0
Total FY 2002 budget request$170,177
FY 2002 forecast from 2001$163,618
% change from forecast4.0%
Reason for change in estimated budget

Administrative office equipment acquisition

Reason for change in scope

N/A

Cost sharing
OrganizationItem or service providedAmountCash or in-kind

Reviews and recommendations

This information was not provided on the original proposals, but was generated during the review process.

Recommendation:
Fundable only if response is adequate
Date:
Sep 28, 2001

Comment:

Response required. This proposal is for monitoring and evaluation of progeny of the captive brood stock collected and reared under project #199801001. The proposal involves co-ordination with state and federal agencies, assistance in the monitoring and evaluation of juveniles and brood adults reared at Bonneville Hatchery and Manchester Marine Laboratory, monitoring and evaluation of the F1 generation juveniles and returning adults, and reporting. Like proposal #199801001, this is a well-written proposal that focuses on research and evaluation of alternative approaches to supplementation through captive broodstock. The proposal presents a thorough technical background that puts the project in context, the rationale and significance to regional programs is detailed and clear, and project history section includes results to date, with some comparisons between stocks and/or rearing treatments. Objectives are again stated as tasks, and not measurable comparisons or tests, but the intentions in this context are clear.

A response is requested, however, concerning the PIT tagging of the F1 juveniles. Section 8 of this proposal indicates that 8,000 PIT tags are included (Objective 3.1). It is not evident from the text, however, if this is the total number of PIT tags allocated to all three populations (are other PIT tags provided by other sources, etc.), and how are these tags allocated between stocks, treatments, and families? This concern needs to be clarified in this proposal. A statistical basis to the tagging program would clearly strengthen this proposal.

In a past review, there was a question concerning overlap between this program and M&E associated with the conventional hatchery production activities. Our understanding is that these M&E tasks are discrete. However, there is an important question associated with these marking programs. The comparison of natural, conventional, and captive brood production will obviously be based on the extensive use of PIT tags in many of the proposals reviewed. Have the co-managers considered the adequacy of marking rates to compare these three types of spring chinook production, and if so, what level of difference in performance may be detectable? This latter issue is not only relevant to this one proposal, but other NPT proposals have noted methods for estimating the numbers of PIT tags required for comparisons. The NPT may be able to advise on this question.


Recommendation:
High Priority
Date:
Nov 30, 2001

Comment:

This project is considered BASE by NMFS.
Recommendation:
Fund
Date:
Dec 21, 2001

Comment:

Fundable. The response comprehensively addresses the review comments. This proposal is for monitoring and evaluation of progeny of the captive brood stock collected and reared under project #199801001. The proposal involves co-ordination with state and federal agencies, assistance in the monitoring and evaluation of juveniles and brood adults reared at Bonneville Hatchery and Manchester Marine Laboratory, monitoring and evaluation of the F1 generation juveniles and returning adults, and reporting. Like proposal #199801001, this is a well-written proposal that focuses on research and evaluation of alternative approaches to supplementation through captive broodstock. The proposal presents a thorough technical background that puts the project in context, the rationale and significance to regional programs is detailed and clear, and project history section includes results to date, with some comparisons between stocks and/or rearing treatments. Objectives are again stated as tasks, and not measurable comparisons or tests, but the intentions in this context are clear.

In a past review, there was a question concerning overlap between this program and M&E associated with the conventional hatchery production activities. Our understanding is that these M&E tasks are discrete. However, there is an important question associated with these marking programs. The comparison of natural, conventional, and captive brood production will obviously be based on the extensive use of PIT tags in many of the proposals reviewed. Have the co-managers considered the adequacy of marking rates to compare these three types of spring chinook production, and if so, what level of difference in performance may be detectable? This latter issue is not only relevant to this one proposal, but other NPT proposals have noted methods for estimating the numbers of PIT tags required for comparisons. A statistical basis to the tagging program would clearly strengthen this, and related, proposals.


Recommendation:
Date:
Feb 1, 2002

Comment:

Statement of Potential Biological Benefit to ESU
Reduce short-term extinction or extirpation risks for the Lostine River.

Comments
Continuing program. Opportunity should be taken to assess monitoring aspects and their relationship to FCRPS Biop. Should benefit from RPA Action Item 176.

Already ESA Req? No

Biop? No


Recommendation:
A
Date:
Feb 11, 2002

Comment:

Recommend as part of larger Grande Ronde supplementation project. Agree with need for review of the adequacy of marking rates to compare the three types of spring Chinook production, and if so, what level of difference in performance may be detectable.

BPA RPA RPM:
--

NMFS RPA/USFWS RPM:
Base


Recommendation:
Fund
Date:
Apr 19, 2002

Comment:

Council Recommendation:

The Nez Perce project implements and evaluates the captive broodstock program through the collection of juvenile salmon from the wild and maintaining them in captivity and releasing the subsequent generation in the wild. The project sponsors sought additional funds above the FY01 base to provide additional staffing for the captive spawning and evaluation of increasingly larger data sets.

ISRP supported the project but made the following observation: "The comparison of natural, conventional, and captive brood production will obviously be based on the extensive use of PIT tags in many of the proposals reviewed. Have the co-managers considered the adequacy of marking rates to compare these three types of spring chinook production, and if so, what level of difference in performance may be detectable?" Bonneville echoed these comments, but supported the project as fundable. NMFS considered the project as BASE for Biological Opinion implementation.

This project is subject to programmatic issue 12. The Council has provided guidance and conditions for funding these types of projects for several years. The Council established general conditions on its funding recommendations for these types of projects in its Fiscal Year 2000 recommendations that remain applicable. The above project, and others (Salmon issue 1) that may be developed: (1) should be funded only to maintain the scope and intensity of existing, previously Council recommended, activities until a basinwide prioritization of target populations and programs is developed (no expansion or new starts); (2) must demonstrate how they are consistent with the policies and standards in the Artificial Production Review report of 2000; (3) must address the project specific issues raised by the ISRP Mountain Snake Province Review Recommendations (2001 12-A); document how the projects address the elements of Captive Propagation Technology in Recovery of Anadromous Salmonids Listed Under the Endangered Species Act (NMFS 1999), outlining steps that sponsors should proceed through when sponsoring a captive propagation initiative.

The Council supports the request for the additional funding. Because the additional funds are for tasks and objectives that are within the existing scope, the Council is able to recommend them under the programmatic issue conditions. The Council recommends increasing the budget for Fiscal Year 2002 by $14,330 over the base budget. The Council believes that the sponsor should address the ISRP project-specific question on marking rates, as well as the programmatic issues related to consistency the APR and the NMFS Captive propagation document (NMFS 1999) in its the submission of the M&E phase for the Northeast Oregon Hatchery as part of its three-step review (see Grande Ronde Issue 2).


Recommendation:
Fund
Date:
Jun 13, 2002

Comment:

Fund conditioned on answering ISRP's questions for review of the adequacy of marking rates to compare the three types of spring chinook production, and if so, what level of difference in performance may be detectable. In addition address the programmatic issues related to consistency with the APR, and the NMFS Captive propagation document (NMFS 1999) in its submission of the M&E phase for the Northeast Oregon Hatchery as part of its three step review.
Recommendation:
Fund
Date:
Sep 20, 2003

Comment:

FY05 represents a 3.4% increase. Programmatic Grande Ronde issue #9.
Recommendation:
Date:
Sep 20, 2003

Comment:

The "FY 03 Obligated" figure does not include the $18,000 for PIT tag costs or the $7,274 carried forward as contract balance.
REVIEW:
NW Power and Conservation Council's FY 2006 Project Funding Review
Funding category:
expense
Date:
May 2005
FY05 NPCC start of year:FY06 NPCC staff preliminary:FY06 NPCC July draft start of year:
$175,620 $175,620 $175,620

Sponsor comments: See comment at Council's website