FY 2001 Ongoing proposal 199305600

Section 1. General administrative information
Section 2. Past accomplishments
Section 3. Relationships to other projects
Section 4. Budgets for planning/design phase
Section 5. Budgets for construction/implementation phase
Section 6. Budgets for operations/maintenance phase
Section 7. Budgets for monitoring/evaluation phase
Section 8. Budget summary

Reviews and Recommendations

Additional documents

Title	Type

Section 1. Administrative

Proposal title	Assessment of Captive Broodstock Technology
Proposal ID	199305600
Organization	National Marine Fisheries Service (NMFS)
Proposal contact person or principal investigator
Name	Dr. Barry Berejikian
Mailing address	P. O. Box 130 Manchester, WA 98353
Phone / email	2068428365 / [email protected]
Manager authorizing this project	Dr. Robert Iwamoto
Review cycle	FY 2001 Ongoing
Province / Subbasin	Systemwide / Systemwide
Short description	Develop captive broodstock husbandry practices through experimental research to i) improve juvenile quality, ii) improve adult reproductive performance, iii) improve in-culture survival, and iv) identify genetic risk factors.
Target species

Project location

Latitude	Longitude	Description

Reasonable and Prudent Alternatives (RPAs)

Sponsor-reported:

RPA

Relevant RPAs based on NMFS/BPA review:

Reviewing agency	Action #	BiOp Agency	Description

Section 2. Past accomplishments

Year	Accomplishment
1995	Reviewed the status of captive broodstock technology with respect to physiology, microbiology, morphology, genetics, and husbandry and produced a comprehensive written document.
1995	Determined differences in reproductive behavior and success of captively reared and wild adult salmon
1996	Developed method to bioencapsulate therapeutic levels of three antibiotic compounds into live adult Artemia.
1996	Demonstrated that hormone implants effectively advanced and synchronized spawning without impairing gamete quality in sockeye salmon
1996	Demonstrated that increased body fat levels were correlated with the onset of early (age-2) male maturity in spring chinook salmon
1996	Developed new DNA microsatellite techniques for determining individual reproductive success of spawning salmon
1997	Demonstrated that growth/body size more important than fat level in affecting early male maturity, and that high growth rate impairs cellular mediated immunity in chinook salmon
1997	Quantified individual breeding success of captively reared adults to determine correlations between morphometric variation and breeding success
1997	Demonstrated that combinations of dietary carotenoid pigments are more effective than single carotenoid supplements in developing appropriate nuptial coloration patterns
1997	Determined the optimum dietary fatty level and feeding level for sockeye salmon reared from fry to spawning, and demonstrated that there was no effect of dietary fat level on precocious maturation, unlike reports in spring chinook salmon
1997	Demonstrated higher and more consistent tissue levels of erythromycin in first feeding salmonids fed live bioencapsulated Artemia diets than conventional medicated pellets.
1998	Developed a freeze-dried bioencapsulated Artemia diet that maintains high palatability with first-feeding salmon. Freeze-dried bioencapsulated Artemia can be produced off-site, shipped and stored until needed
1998	Determined the effects of male size on female breeding behavior
1998	Provided evidence that early survival in chinook salmon can be sensitive to modest levels of inbreeding (12-25% greater than a control) incurred in a single generation
1998	Shown that chinook salmon body size and shape are highly heritable and have similar genetic and phenotypic architectures, suggesting that selection could act quickly to affect variation in these characters
1998	Demonstrated that resistance to infection by Vibrio in chinook salmon is heritable but that time to death is not. These results indicate the importance in captive broodstock programs of considering the genetic bases of traits that affect fitness
1998	Quantified individual reproductive success of captively reared adults to determine correlations between morphometric variation and breeding success. Demonstrated that kype length is correlated with behavioral measures of male breeding success.
1998	Demonstrated that diets supplemented with krill and vitamins reduced embryonic deformities in sockeye salmon.
1998	Demonstrated that rearing temperature decreased age-at-maturity in sockeye salmon
1998	Developed a panel of assays to assess immune functions of salmonid broodstock reared in captivity
1998	Completed the first quantitative profile of immune functions of sockeye salmon reared at different temperatures, and identified a rearing temperature effect on antibody responses.
1998	Demonstrated that azithromycin decreased mortality caused by Bacterial Kidney Disease, when compared to conventional (erythromycin) treatments.
1999	Quantified the effects of one consequence of early male maturation (i.e., male size) on female breeding behavior and success . Demonstrated that females respond less favorably to smaller males by delaying spawning.
1999	Directly evaluated the breeding success of captively reared adults released to spawn in their natal streams in Idaho. This research has lead to developing more effective monitoring plans and protocols, and guided reintroduction strategies
1999	Described the detailed reproductive behavior patterns of chinook salmon. This information (previously absent from the published literature) guided monitoring and evaluation efforts for programs released ESA-listed, captively reared adult chinook salmon.
1999	Described the detailed reproductive behavior patterns of chinook salmon and developed protocols for use in monitoring breeding behavior of ESA-listed chinook salmon
1999	Directly evaluated the breeding success of captively reared adults released to spawn in their natal streams in Idaho
2000	Recorded survival and growth data from brood year 1997 and 1998 inbred progeny of captively reared chinook salmon

Section 3. Relationships to other projects

Project ID	Title	Description

Section 4. Budget for Planning and Design phase

Task-based budget

Objective	Task	Duration in FYs	Estimated 2001 cost	Subcontractor
No objectives for Planning and Design			$0

Outyear objectives-based budget

Objective	Starting FY	Ending FY	Estimated cost

Outyear budgets for Planning and Design phase

Section 5. Budget for Construction and Implementation phase

Task-based budget

Objective	Task	Duration in FYs	Estimated 2001 cost	Subcontractor
1. Reduce mortality from Bacterial Kidney Disease (BKD) in chinook salmon	a. Evaluation of toxic effects of long-term prophylactic use of azithromycin and erythromycin in fall chinook salmon	2	$55,400
1.	b. Determine azithromycin depuration or inactivation rates in effluent from salmon during feed-based antibiotic treatment.	2	$60,100
1.	c. Determine efficacy of vaccines	3	$97,750
2. Improve adult salmon natural reproductive performance	a. Evaluate the effects of rearing environments and acclimation on reproductive behavior and success	4	$198,840
2.	b. Evaluate the effects of hydraulic embryo sampling on viability	2	$92,600
3. Improve reintroduction success	a. Assess relative success of different re-introduction (release) strategies	4	$106,200
4. Improve physiological development including maturation synchrony and reproductive characteristics	a. Evaluate the effects of growth rate on incidence of early male maturity and adult quality in spring chinook salmon	4	$195,650
4.	b. Evaluate the effects of growth on maturation timing, fecundity, egg size, and egg quality.	4	$195,200
5. Reduce genetic impacts of captive breeding programs	a. Determine effects of close inbreeding on captive and released chinook salmon	3	$68,460
5.	b. Characterize genetic basis of life history in chinook salmon	3	$67,500	Yes
6. Enhance juvenile body characteristics of salmon	a. Assess the quality of fish released from ongoing captive broodstock program	3	$48,000	Yes
6.	b. Evaluate the effects of dietary protein and energy intakes on post-release fitness.	3	$52,000	Yes
7. Improve olfactory imprinting in sockeye salmon	a. Determine environmental effects on imprinting at different developmental stages	5	$162,500	Yes

Outyear objectives-based budget

Objective	Starting FY	Ending FY	Estimated cost

Outyear budgets for Construction and Implementation phase

FY 2004	FY 2005	FY 2002	FY 2003
$1,529,800	$1,575,700	$1,442,000	$1,485,300

Section 6. Budget for Operations and Maintenance phase

Task-based budget

Objective	Task	Duration in FYs	Estimated 2001 cost	Subcontractor

Outyear objectives-based budget

Objective	Starting FY	Ending FY	Estimated cost

Outyear budgets for Operations and Maintenance phase

Section 7. Budget for Monitoring and Evaluation phase

Task-based budget

Objective	Task	Duration in FYs	Estimated 2001 cost	Subcontractor

Outyear objectives-based budget

Objective	Starting FY	Ending FY	Estimated cost

Outyear budgets for Monitoring and Evaluation phase

Section 8. Estimated budget summary

Itemized budget

Item	Note	FY 2001 cost
Personnel	FTE: NMFS	$227,352
Fringe	NMFS	$50,699
Supplies		$210,000
Travel	Air, gov't vehicle, ferry, and POV	$28,740
Indirect	SLUC, NOAA	$139,068
Capital	Freshwater captive broodstock rearing and experimental evaluation facilities	$48,000
PIT tags	# of tags: 4000	$9,200
Subcontractor	University of Washington	$329,240
Subcontractor	University of Idaho	$87,982
Subcontractor	Frank Orth and Associates	$48,300
Subcontractor	Miscellaneous Service Contracts	$26,000
Subcontractor	Pacific States Marine Fisheries Commission	$162,839
Other	Rents, Communication, Utilities, Printing	$32,780
		$1,400,200

Total estimated budget

Total FY 2001 cost	$1,400,200
Amount anticipated from previously committed BPA funds	$0
Total FY 2001 budget request	$1,400,200
FY 2001 forecast from 2000	$1,400,000
% change from forecast	0.0%

Reason for change in estimated budget

No change

Reason for change in scope

In February 1999, project coordinators consulted with the Stanley Basin Sockeye and Snake River Chinook Technical Oversight Committees, including the project's BPA COTR, to assist in prioritizing research objectives. As a result Objective 5, Improve Olfactory Imprinting in Sockeye Salmon, was identified as critical to the success of the sockeye salmon captive broodstock program and was added to this research project. Other lower priority objectives were phased-out to accommodate this research. The remaining Objectives may appear different from the fy2000 proposal. This is because the project outline has been re-organized to better communicate the Objectives, Tasks, and products of the project in response to the fy2000 ISRP project review .

Cost sharing

Organization	Item or service provided	Amount	Cash or in-kind

Reviews and recommendations

This information was not provided on the original proposals, but was generated during the review process.

REVIEW:
CBFWA Funding Recommendation

Recommendation:
Ongoing Funding: yes; New Funding: no

Date:
Jul 14, 2000

Comment:

There is no change in budget.

In February 1999, project coordinators consulted with the Stanley Basin Sockeye and Snake River Chinook Technical Oversight Committees, including the project's BPA COTR, to assist in prioritizing research objectives. As a result Objective 7, Improve Olfactory Imprinting in Sockeye Salmon, was identified as critical to the success of the sockeye salmon captive broodstock program and was added to this research project. Other lower priority objectives were phased-out to accommodate this research. The remaining objectives may appear different from the FY 2000 proposal. This is because the project outline has been reorganized to better communicate the objectives, tasks, and products of the project in response to the FY 2000 ISRP project review. The MainSys SRT supports the reorganization of tasks and objectives.

REVIEW:
NWPPC Funding Recommendation

Recommendation:
Fund

Date:
Sep 13, 2000

Comment:

Rationale: Budget increase inappropriate in this review.

REVIEW:
NW Power and Conservation Council's FY 2006 Project Funding Review

Funding category:
expense

Date:
May 2005

FY05 NPCC start of year:	FY06 NPCC staff preliminary:	FY06 NPCC July draft start of year:
$1,468,100	$1,468,100	$1,468,100

Sponsor comments: See comment at Council's website